TABLE 1.
Bacterial strains, their sources, and media used for their cultivation
| Species | Sourcea | Mediumb | PCR productc
|
|
|---|---|---|---|---|
| 0.7 kb | 0.4 kb | |||
| Bacteroides fragilis | MMB S16 | WW | − | ND |
| Bacteroides thetaiotaomicron | MMB S20 | WW | − | ND |
| Bifidobacterium lactis Bb12 | VTT | WW | − | ND |
| Butyrivibrio fibrisolvens-like clone A11 | LB | − | ND | |
| Clostridium beijerinckii | MMB 3318 | WW | − | ND |
| Clostridium bifermentans | NIZO B529 | WW | − | ND |
| Clostridium celerescens-like clone A54 | LB | − | ND | |
| Coprococcus eutactus-like clone A19 | LB | − | ND | |
| Enterococcus faecalis | DSM 20478 | WW | − | ND |
| Escherichia coli JM109 | Promega | LB | − | ND |
| Eubacterium biforme-like clone F44 | LB | ND | − | |
| Eubacterium halii-like clone A07 | LB | − | − | |
| Eubacterium rectale-like clone A13 | LB | − | − | |
| Fusobacterium prausnitzii-like clone A03 | LB | − | ND | |
| Fusobacterium prausnitzii-like clone A10 | LB | − | ND | |
| Lactococcus lactis subsp. lactis | NIZO B17 | MRS | − | ND |
| Lactobacillus acidophilus | VTT E96276 | MRS | + | + |
| Lactobacillus brevis | VTT E91458 | MRS | + | + |
| Lactobacillus buchneri | VTT E93445 | MRS | + | + |
| Lactobacillus curvatus | VTT E90391 | MRS | + | + |
| Lactobacillus fermentum | VTT E93489 | MRS | + | + |
| Lactobacillus helveticus | ATTC 15009 | MRS | + | + |
| Lactobacillus helveticus | NCIMB 8652 | MRS | + | + |
| Lactobacillus paracasei F19 | VTT | MRS | + | + |
| Lactobacillus plantarum | VTT E79098 | MRS | + | + |
| Lactobacillus reuteri | VTT E92142 | MRS | + | + |
| Lactobacillus rhamnosus LGG | ATCC 53103 | MRS | + | + |
| Lactobacillus ruminis | VTT E91470 | MRS | + | + |
| Lactobacillus salivarius subsp. salicinius | DSM 20554 | MRS | + | + |
| Leuconostoc sp. | NIZO B179 | WW | + | + |
| Pediococcus sp. | FM 0001 | MRS | + | + |
| Peptostreptococcus anaerobius | MMB 2828 | WW | − | ND |
| Roseburia cecicola-like clone A22 | LB | − | ND | |
| Ruminococcus obeum-like clone A20 | LB | − | ND | |
| Streptococcus thermophilus | NIZO B119 | WW | − | ND |
| Vagococcus fluvialis | DSM 5731 | WW | − | ND |
| Weissella kandleri | DSM 20593 | WW | + | ND |
MMB, Department of Medical Microbiology, University of Groningen, 9713 GZ Groningen, The Netherlands. VTT, VTT Culture Collection, FIN-02044, Finland. NIZO, Netherlands Institute for Dairy Research, 6710 BA Ede, The Netherlands. DSM, German Collection for Microorganisms, 3300 Braunschweig-Stöckheim, Germany. ATCC, American Type Culture Collection, Rockville, Md. NCIMB, National Collection of Industrial, Food, and Marine Bacteria, Aberdeen, AB24 3RY, Scotland, United Kingdom. FM, Food Microbiology, Wageningen University, 6703 HD Wageningen, The Netherlands.
WW, Wilkins West; LB, Luria-Bertani broth; MRS, Lactobacillus MRS broth.
The formation of specific PCR products with a size of 0.7 kb by using the primer set Bact-0011f and Lab-0677r or of 0.4 kb by using the primer set Lab-0159f and Uni-0515r at an annealing temperature of 66°C is also indicated (+, present; −, absent). ND, not determined.