TABLE 2.
Primers used in this study
| Primera | Primer sequence (5′-3′) | Source or reference |
|---|---|---|
| S-D-Bact-0011-a-S-17 | AGA GTT TGA T (C/T) (A/C) TGG CTC AG | 16 |
| S-D-Bact-0124-a-S-27 | GGA CGG GTG AGT AAC ACG | 18 |
| S-D-Lab-0158-a-S-20 | TGG AAA CAG (A/G) TG CTA ATA CC | 11 |
| S-G-Lab-0159-a-S-20 | GGA AAC AG (A/G) TGC TAA TAC CG | This study |
| S-*-Univ-0515-a-A-24 | ATC GTA TTA CCG CGG CTG CTG GCA | 18 |
| S-G-Lab-0677-a-A-17 | CAC CGC TAC ACA TGG AG | This study |
| S-G-Lacb-0722-a-A-25 | (C/T) CA CCG CTA CAC ATG (A/G) AG TTC CAC T | 39 |
| GC-clamp | CGC CGG GGG CGC GCC CCG GGC GGG GCG GGG GCA CGG GGG G | 29 |
| T7 | TAA TAC GAC TCA CTA TAG G | Promega |
| Sp6 | GAT TTA GGT GAC ACT ATA G | Promega |
a
Apart from the GC clamp and primers T7 and Spb, the nomenclature is according to Alm et al. (2).