TABLE 3.
Alignment of primer S-G-Lab-0677-a-A-17 with target sequences of 16S rDNA (region 677 to 693 of E. coli numbering) from Lactobacillus and other species
| Primer or species (no. of species found)a | Sequence |
|---|---|
| S-G-Lab-0677-a-A-17 reverse primer | 5′-C ACC GCT ACA CAT GGA G-3′ |
| All Lactobacillus spp. subgroupsb (94) | GTA AGG TGG CGA TGT GTA CCT CAA G |
| All Leuconostoc spp. (18) | GTA AGG TGG CGA TGT GTA CCT CAA G |
| All Weissella spp. (16) | GTA AGG TGG CGA TGT GTA CCT CAA G |
| All Pediococcus spp. (12) | GTA AAG TGG CGA TGT GTA CCT CAA G |
| Bacteroides fragilis | GTA AAG TGG CGA TGT GGTGCT TAA G |
| Bacteroides vulgatus | GTA AAG TGG CGA TGT GGTGCT TAA G |
| Bifidobacterium bifidum | GTA AGG TGG CAA TGT GGC CCT TAA G |
| Clostridium coccoides | GTA AAG TGG CGA TGT GAT CCT TAA G |
| Clostridium perfringens | GTA AAG TGG CGA TGT GAT CCT TAA G |
| Clostridium ramosum | GTA AAG TGG CGA TGT GTA CCT TAA G |
| Enterococcus faecalis | GTA AAG TGG CGA TGT GTA CCT TAA G |
| Eubacterium biforme | GTA AAA TGG CGA TGT GTA CCT CAA G |
| Eubacterium rectale | GTA AAG TGG CGA TGT GAT CCT TAA G |
| Lactococcus lactis | GTA AAG TGG CGA TGT GTA CCT TAA G |
| Staphylococcus aureus | GTA AAG TGG CGA TGT GTA CCT TAA G |
| Streptococcus thermophilus | GTA AAG TGG CGA TGT GTA CCT TAA G |
| Vagococcus fluvialis | GTA AAG TGG CGA TGT GTA CCT TAA G |
a
The number of species found in the database is indicated in parentheses. The mismatches with the primer sequence are indicated in boldface and are underlined.
b
Includes all Lactobacillus species in GenBank, except Lactobacillus maltaromicus, L. catenaformis, and L. vitulinus.