FIG. 2.

PCR analysis of pAPGUSNNB transformants to detect 3′ and 5′ nor-1 integration. Genomic DNA was isolated from A. parasiticus and amplified by PCR using the primers (Table 1) represented in Fig. 1 by arrows (PCR 1 [5′ nor-1] and PCR2 [3′ nor-1]). (A) PCR analysis of 3′ nor-1 integrants of pAPGUSNNB (transformants 50, 59, 61, 68, 85, and 96). (B) PCR analysis of 5′ nor-1 integrants (transformants 8, 34, 67, and 73). NR-1 (parent strain) and transformants 10 (pAPGUSNNB integrated at niaD) and 15 (site of integration not completely defined) were used as negative controls.