FIG. 3.

Real-time quantitative PCR analysis of urease A gene expression of 4-, 20-, 40-, and 60-day broth-cultured and 3- and 28-month cold-starved H. pylori. (A and B) Real-time amplification curve (A) and melting-curve (B) analysis of amplified PCR products, using SYBR Green I and the LightCycler. (C) Agarose gel electrophoresis. Lanes 1 and 2, water control; lanes 3 and 4, 4-day cells; lanes 5 and 6, 20-day cells; lanes 7 and 8, 40-day cells; lanes 9 and 10, 60-day cells; lanes 11 and 12, 3-month cells; lanes 13 and 14, 28-month cells. The experiments were done in duplicate.