TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or phenotype | Source |
|---|---|---|
| Synechococcus sp. strain MA 19 | Thermophilic, optimal growth at 50°C | NIBHTa |
| Escherichia coli strains | ||
| TOP10 | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 ΔlacU169 (φ80 lacZΔM15) | Invitrogen (San Diego, Calif.) |
| XL1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lac1qZΔM15Tn10(Tetr)] | Stratagene |
| Plasmids | ||
| pBluescript SK(−) | Amprlac POZ′, T7 and T3 promotor | Stratagene |
| pSK::H6.5 | 6.5-kbp HindIII fragment from Synechococcus sp. strain MA19 genomic DNA harboring complete cphB and 5′ part of cphA inserted in pBluescript SK(−) | This study |
| pSK::Kp1.7 | 1.7-kbp inverse PCR product from Synechococcus sp. strain MA19 genomic DNA containing 3′ region of cphA inserted in pBluescript SK(−) | This study |
| pSK::cphAMA19co | 2.98-kbp PCR product from Synechococcus sp. strain MA19 genomic DNA carrying cphA inserted in pBluescript SK(−) colinear with respect to lacPO | This study |
| pSK::cphAMA19anti | 2.98-kbp PCR product from Synechococcus sp. strain MA19 genomic DNA carrying cphA inserted in pBluescript SK(−) antilinear with respect to lacPO | This study |
a
NIBHT, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, Japan.