TABLE 2.
Expression of Synechococcus sp. strain MA19 cphA and accumulation of cyanophycin in recombinant E. colia
| Plasmid | Orientationb | IPTGc | Cyanophycin content (% [wt/wt] of cell dry matter) | Sp actd |
|---|---|---|---|---|
| pSK::cphAMA19co | Colinear | + | 25.0 ± 2.0 | 1.81 ± 0.1 |
| − | 11.5 ± 2.0 | 0.66 ± 0.1 | ||
| pSK::cphAMA19anti | Antilinear | + | 0.5 ± 0.2 | 0.05 ± 0.1 |
| − | 0.5 ± 0.1 | 0.05 ± 0.1 | ||
| pBluescript SK(−) | Not relevant | + | 0.1 ± 0.1 | 0.05 ± 0.1 |
a
The experiments and enzyme assays were done in triplicate, and the standard errors were calculated by using standard statistics (P < 0.05).
b
Orientation with respect to lacPO.
c
Recombinant E. coli TOP10 cells were grown in Luria-Bertani medium in the presence (+) or in the absence (−) of 1.0 mM IPTG as described in Materials and Methods.
d
Specific activity is expressed as the rate of incorporation of l-arginine in nanomoles per minute per milligram of protein.