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Mutations in the pocket of ankyrin repeat 1 abolish assembly of the RFX complex. The RFXANK mutant containing substitutions in the ankyrin groove region of repeat 1 (pocket mutant P1) was analyzed in the complementation (A), coimmunoprecipitation (B), and GST pull-down (C) assays as described in the legends to Fig. 1 and 5. wt, wild type. (D) Space-filling model of the RFXANK ARD. The surface implicated in RFX complex assembly is marked in gray.
