FIG. 1.

Isolation of a Lin⁻ CD41⁺ FcγR^low c-kit⁺ CD9⁺ progenitor cells from mouse E13.5 to E14.5 fetal liver. (a) Flow cytometric analysis of Lin⁻IL-7R⁻Sca-1⁻ wild-type and ΔneoΔHS fetal liver cells labeled with CD41-fluorescein isothiocyanate, FcγR-PE, c-kit-APC, CD9-biotin, and streptavidin-APC-Cy7. Sorting gates for isolation of Lin⁻ CD41⁺ FcγR^low c-kit⁺ CD9⁺ cells are shown. (b) The table shows the average frequencies of Lin⁻ CD41⁺ FcγR^low c-kit⁺ CD9⁺ population in wild-type and ΔneoΔHS fetal liver from nine different sorting experiments. Note that only 10% of total fetal liver cells are Lin⁻.