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The PERE element affects both basal and PE-induced promoter activity. (A) Schematic representation of the −695 ANF promoter with the sequence of the wild-type (Wt) and mutated PERE. (B) Cardiomyocytes were transfected with wild-type and mutated PERE −695 ANF promoter luciferase reporter constructs and stimulated for 48 h with 0.1 mM PE. The data shown represent the mean ± the SEM of at least six independent determinations.
