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. 2005 Oct;25(19):8669–8682. doi: 10.1128/MCB.25.19.8669-8682.2005

FIG. 3.

FIG. 3.

Expression and regulation of PEX1 mRNA. (A) PEX1 and GAPDH mRNA levels were analyzed by Northern blot in total RNA isolated from the heart at E14 and from adult heart, as well as from primary cardiomyocyte cultures prepared from neonatal (1- and 4-day-old) rats. (B) Tissue distribution of PEX1 mRNA on total RNA from adult rat tissues. V, ventricles; Lu, lung; S, stomach; K, kidney; B, brain; Li, liver; M, skeletal muscle; A, adrenal glands; T, testis. (C) PEX1, ANF, and tubulin-α mRNA levels were determined by reverse transcription-PCR with total RNA extracted from cardiomyocytes isolated from 4-day-old rats treated or not treated with PE for 6 h. (D) PEX1, ANF, and GAPDH mRNA levels were analyzed by Northern blotting with total RNA extracted from cardiomyocytes isolated from 4-day-old rats treated with PE for longer times. Veh, vehicle.