FIG. 5.

Stability of cystatin C, Rab11a, or RANKL is unaltered in PDK-1^+/+ and PDK-1^−/− ES cells. (A) PDK-1^+/+ and PDK-1^−/− ES cells were treated with 20 μg of cycloheximide (CHX)/ml for the indicated times. After harvesting the cells, 10 μg of soluble proteins were separated by SDS-PAGE and transferred to PVDF membranes. Immunoblotting with antibodies against cystatin C, Rab11a, and RANKL was performed. (B) The protein levels of cystatin C after the cycloheximide treatment in panel A were quantitated and are shown graphically.