FIG. 1.
Endogenous USF1 and USF2 inhibit hCYP19 gene expression in JEG3 cells. JEG3 cells were transfected with USF1 siRNA, USF2 siRNA, USF1 siRNA plus USF2 siRNA or with scrambled siRNA, as a control, and cultured for an additional 48 h. hCYP19 mRNA levels were analyzed by Northern blotting using a full-length 32P-labeled hCYP19 cDNA as probe. The blot was reprobed for 28S rRNA as a control for RNA loading and transfer. USF1 and USF2 protein levels in lysates from the same cells were analyzed by immunoblotting (two lower panels).