FIG. 6.

Proteasome inhibitors prevent fusion and differentiation of cultured human trophoblasts. Freshly isolated cytotrophoblasts were cultured in a 20% O₂ atmosphere in the absence or presence of proteasome inhibitors for 16 h (A). After 16 h, the medium in some dishes was removed; fresh medium without proteasome inhibitors was then added, and the cells were cultured for an additional 16 h (B). Shown are light micrographs of hematoxylin- and eosin Y-stained cells (magnification, ×200).