FIG. 3.

Ap₄A mediates the dissociation of USF2/Hint complex. (A) The GST-Hint protein bound to glutathione-Sepharose beads was incubated with [³⁵S]methionine-labeled USF2 overnight at 4°C. The USF2/Hint protein complex was exposed to Ap₂A, Ap₃A, Ap₄A, and Ap₅A compounds for 1 h at 37°C. One representative result of three is shown. (B) Densitometry analysis. The bands shown in panel A were subjected to densitometry analysis, and the results were normalized to GST control. (C) Dose-dependent dissociation of USF2/Hint protein complex by Ap₄A. Increasing amounts of Ap₄A were added to the USF2/Hint protein complex, and a pulldown assay was carried out. One representative result of three is shown. (D) Densitometry analysis. The bands shown in panel C were subjected to densitometry analysis, and the results were normalized to the GST control.