FIG. 5.

Immunological activation and Ap₄A show positive effects on the transcript levels of USF2 target genes. (A) Expression level of USF2 target genes in IgE- and DNP-activated RBL cells. The RBL cells were incubated with 50-ng/ml IgE overnight, and then the cells were incubated 0, 6, 10, 20, and 28 h in the presence of DNP. The mRNA levels of CXCR4, FceRI, APC, TSP-1, TERT, SHP, TGFb2, TGFb1, and TGFb3 genes were measured and normalized to control. For each gene, the time point with the highest increase in the transcript level is shown. One representative result of three is shown. (B) Expression of the USF2 target genes in unstimulated RBL cells treated with 100 μM (each) Ap₄A, Ap₃A, and AMP. RNA from RBL cells was collected 12 h after the administration of Ap₄A, Ap₃A, and AMP. Values show the severalfold increase of the transcript levels normalized to that of the control. One representative result of three is shown.