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. 2025 Nov 26;16:2173. doi: 10.1007/s12672-025-03989-9

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 10 — Interference with the expression of FSTL1 can inhibit the proliferation, invasion, and migration of colon cancer cells. A qRT-PCR was used to detect the expression level of FSTL1 in normal colon epithelial cells and colon cancer cell lines. B, C The knockout efficiency of FSTL1 in colon cancer cells SW480 and HCT116 was detected by qRT-PCR. D, E CCK8 assay showed that knockout of FSTL1 gene inhibited the proliferation of colon cancer cells SW480 and HCT116. Knockout of the F, I FSTL1 gene inhibits the migration and invasion ability of colon cancer cells SW480 and HCT116. *p<0.05;**p<0.01;***p<0.001;NS: Not statistically significant