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. 2025 Nov 26;16:2173. doi: 10.1007/s12672-025-03989-9

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 5 — The mutation status ofFSTL1 in colorectal cancer and its correlation with immune checkpoints. A The cBioPortal tool was utilized to display the mutation frequency of FSTL1 across four colorectal cancer cohorts. B A heatmap illustrates the correlation between FSTL1 and the CTL immune cohort. C–E Demonstrates the correlation between FSTL1 and three immune checkpoint molecules. F–G Shows the methylation levels ofFSTL1 in colorectal cancer.