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. 2005 Oct;25(20):8971–8984. doi: 10.1128/MCB.25.20.8971-8984.2005

FIG. 7.

FIG. 7.

Analysis of the localization of SCC1/DRAD21 on chromosomes after depletion of Barren/CAP-H. Both control and Barren/CAP-H-depleted (96 h) cells were immunostained for POLO and DRAD21. (A) Cells arrested at metaphase by 2 h incubation with 20 μM MG132. In control cells SCC1/DRAD21 localizes between sister chromatids as a tight line between sister centromeres. However, after depletion of Barren/CAP-H, SCC1/DRAD21 is distributed over a broad area between sister centromeres. (B) Cells were incubated with 30 μM colchicine for 2 h to arrest them at prometaphase before microtubules could bind kinetochores. In these cells, SCC1/DRAD21 localizes to a thin line between sister centromeres in both control and Barren/CAP-H-depleted chromosomes. Scale bars are 5 μm.