TABLE 2.

Meiotic DSBs at M26/CRE sites in wild-type S. pombe

Site	Enzyme used for DNA digestion	Unbroken fragment sizea(kb)	Predicted size of broken fragmentb(kb)	Break ≤1 kb from predicted positionc	Break ≥1 kb from predicted positionc	% breakage pcr1+d	% breakage pcr1Δd
1-1	PacI	15.2	10.4	+		0.5	0.5
1-1	PacI	15.2	10.4		+	2.3	1.1
1-2	NruI	15.0	10.8
1-3	NruI	17.5	14.4	+		3.2, 2.7	2.3, 2.2
1-4	NcoI	9.5	7.2	+		7.0	5.0
2-1	PacI	15.9	12.4	+		3, 4.3	1.5, 2.8
2-2	PacI	8.4	5.2		+	1.9	0.6
2-3	PacI	18.3	13.1	+		3.3, 5.0	1.8, 2.9
2-4	NruI	7.9	5.1	+		<0.5	<0.5
2-4	NruI	7.9	5.1		+	<0.5	<0.5
2-5	NruI	8.2	6.2	+		0.8, 0.5	<0.5, <0.5
2-6	NruI	14.0	10.7
2-7	NruI	18.7	12.8		+	<0.5, <0.5	<0.5, <0.5
3-2	NruI	10.3	4.4	+		<0.5, <0.5	<0.5, <0.5
3-3	NruI	28.7	16.0		+	2.0	1.0
3-4	PacI	10.5	6.5	+		1.8, 1.6	0.9, <0.5
3-5	NruI	17.0	8.8	+		3.4	2.1

^aPredicted size of unbroken fragment from S. pombe genomic sequence (http://www.sanger.ac.uk/Projects/S_pombe/).

^bSize of hybridizing fragment predicted from a break within the M26/CRE sequence.

^cFig. 2; see Fig. S1 of the supplemental material.

^dPercentage of total DNA in broken fragments. Pairs of values are from separate blots of one induction. pcr1⁺ and pcr1Δ strains were compared side by side on the same blot. The first value of each pair (pcr1⁺ or pcr1Δ) is from one blot, and the second value is from the second blot.