FIG. 3.

Identification of Gal4 activating region cross-linking targets. Cross-linking was performed on PICs as described in the legend to Fig. 2, except that the indicated Flag-tagged nuclear extracts (NE) were used. The relevant bands in each panel are marked with asterisks. (A) Identification of p48 and p64 as Gal80 and Taf12. (B) Identification of p120 as Gal11. (C) Identification of p80 as Ste12. (D) Identification of Tra1 as the largest cross-linked polypeptide. After cross-linking, PICs were dissociated from the template with a high salt concentration and immunoprecipitated with anti-Flag beads.