TABLE 3.
Results of control hybridizations for CFB-specific 16S rDNA-targeted oligonucleotide probesa
| Species | Catalogue no. | Phylogeny | Hybridization with:
|
|||
|---|---|---|---|---|---|---|
| CFB560 | CFB562 | CFB376 | CF319a/b | |||
| CFBs | ||||||
| Cytophaga fermentans | NCIMB 2218 | Bacteroides | + | + | − | + |
| Bacteroides distasonis | ATCC 8503 | Bacteroides | + | + | − | + |
| Bacteroides fragilis | ATCC 25285 | Bacteroides | + | + | − | − |
| Bacteroides eggerthii | NCTC 11185 | Bacteroides | + | + | − | − |
| Microscilla aggregans | NCIMB 1418 | Cytophaga | + | − | − | + |
| Chryseobacterium indoltheticum | NCIMB 2220 | Cytophaga | + | − | − | + |
| Flavobacterium aquatile | NCIMB 8694 | Cytophaga | + | − | − | + |
| Flexibacter maritimus | NCIMB 2154 | Cytophaga | + | − | − | + |
| Cytophaga uliginosa | NCIMB 1863 | Cytophaga | + | − | − | + |
| Cytophaga latercula | NCIMB 1399 | Cytophaga | + | − | − | + |
| Cytophaga marinoflava | ATCC 19326 | Cytophaga | + | − | − | + |
| Pedobacter heparinus | NCIMB 9290 | Sphingobacterium | + | − | − | + |
| Saprospira grandis | NCIMB 1363 | Lewinella | + | + | + | + |
| Microscilla arenaria | NCIMB 1413 | Persicobacter | + | + | + | − |
| Microscilla sericea | NCIMB 1403 | Cytophaga aurantiaca | + | + | + | + |
| Non-CFBs | ||||||
| Agrobacterium tumefaciens | Putative isolate | α-Proteobacteria | − | − | − | − |
| Burkholderia cepacia | NCTC 10661 | β-Proteobacteria | − | − | − | − |
| Acinetobacter calcoaceticus | NCIMB 8250 | γ-Proteobacteria | − | − | − | − |
| Proteus vulgaris | NCTC 6213 | γ-Proteobacteria | − | − | − | − |
| Pseudomonas fragi | NCTC 10689 | γ-Proteobacteria | − | − | − | − |
| Pseudomonas aeruginosa | Putative isolate | γ-Proteobacteria | − | − | − | − |
| Myxococcus xanthus | NCIMB 9412 | δ-Proteobacteria | − | − | − | − |
| Synechococcus sp. | PCC 7942 | Cyanobacteria | − | − | − | − |
| Synechocystis sp. | PCC 6803 | Cyanobacteria | − | − | − | − |
| Nostoc sp. mac | PCC 8009 | Cyanobacteria | − | − | − | − |
| Nostoc sp. | PCC 7524 | Cyanobacteria | − | − | − | − |
| Anabaena sp. | PCC 7126 | Cyanobacteria | − | − | − | − |
| Bacillus megaterium | ATCC 12872 | Gram positive | − | − | − | − |
| Bacillus cereus | NCTC 7464 | Gram positive | − | − | − | − |
| Bacillus subtilis | NCTC 3610 | Gram positive | − | − | − | − |
| Staphylococcus aureus | NCTC 6571 | Gram positive | − | − | − | − |
| Brevibacter acetylicum | Putative isolate | Gram positive | − | − | − | − |
| Arthrobacter atrocyaneus | Putative isolate | Gram positive | − | − | − | − |
| Clone 83.8% related to Verrucomicrobiales clone CO19 | Verrucomicrobiales | − | − | − | − | |
| Clone 87.8% related to Pirellula marina | Planctomyces | − | − | − | − | |
a
Probes CFB560, CFB562, and CFB376 (this study) and probe CF319a/b (32). PCR-amplified 16S rDNAs from target and nontarget bacteria were fixed onto nylon membrane and hybridized against probes under optimized conditions. The phylogeny of test organisms stated here is as listed in the RDP (release 8.0) small-subunit phylogentic trees.