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. 2005 Oct;25(20):8864–8873. doi: 10.1128/MCB.25.20.8864-8873.2005

FIG. 5.

FIG. 5.

LMCD1/dyxin is retained in the nucleus of leptomycin B-treated cells. 293 cells were transfected with a myc-tagged LMCD1 expression construct and treated with 20 μM leptomycin B for 4 hours. Cells were stained with the anti-myc epitope antibody (9E10). Untreated cells showed primarily cytoplasmic staining of LMCD1/dyxin (A), whereas leptomycin B-treated cells showed an accumulation of protein within the nucleus. (B) Fluorescein isothiocyanate staining of LMCD1; (C) DAPI (4′,6′-diamidino-2-phenylindole) staining; (D) overlay of panels B and C. Coexpression of LMCD1 (E) and GATA6 (F) does not change cellular localization of GATA6. Panel G is an overlay of panels E and F.