FIG. 7.

LMCD1/dyxin does not have inherent transcriptional repression activity, and the full-length protein is required for repression. LMCD1/dyxin, or domains within, does not repress a GAL4-responsive reporter containing the simian virus 40 promoter (A). A GAL4-GATA6 fusion protein was used to activate the GAL4 response reporter, and increasing amounts of LMCD1 were coexpressed. LMCD1/dyxin does not affect the activity of GATA6 when fused to the GAL4 DNA binding domain (B). The indicated domains in LMCD1/dyxin were expressed, along with GATA6 and the SP-A luciferase reporter in 293 cells, to show that full-length LMCD1/dyxin can repress GATA6 activity on the SP-A promoter but that individual domains within LMCD1/dyxin cannot (C). Data represent the averages of results of three assays ± standard errors of the means.