FIG. 1.

Schematic representation of L1Tc and C2-L1Tc purification. (A) Scheme of the L1Tc element; the endonuclease (AP), reverse transcriptase (RT), RNase H (RH) (29), and zinc finger (C₂H₂) domains (21) are separated by thin black lines. The region comprising the C2-L1Tc domain is indicated as a gray box, and the corresponding sequence (the GenBank accession number is indicated at the left of the element) and the Zn finger consensus are shown below the scheme. (B) Coomassie blue staining of one of the preparations of recombinant C2-L1Tc used in NAC activity assays. Lane 1, noninduced TAP-F E. coli cells transformed with the pCAS-C2-L1Tc vector; lane 2, salicylate-induced TAP-F E. coli cells transformed with the pCAS-C2-L1Tc vector; lane 3, C2-L1Tc recombinant protein after Ni²⁺-NTA chromatography; lane 4, purified C2-L1Tc recovered after Superdex 75 fast protein liquid chromatography.