FIG. 7.

Strand exchange assay employing mismatched DNA duplexes. (A) Scheme of the strand exchange assay using 1 nM of 29/*mm29c preformed duplex and a 50-fold molar excess of cold 29c primer. Reactions were performed at 37°C using different concentrations of C2-L1Tc and different periods of incubation (as indicated). The single-strand form of the radiolabeled primer (ss*) and the alternative structure that the free *mm29C primer can adopt (**), as previously described (23), are indicated. (B) Quantification of the released single-stranded ³²P-labeled *mm29c oligonucleotide was performed using ImageQuant software (Pharmacia).