Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Strand exchange assay employing perfect DNA duplexes. Shown are a scheme and results of the strand exchange assay using preformed perfect duplexes with different numbers of paired bases. A total of 1 nM of preannealed *29/25c (A) or *29/29c (B) DNA duplex was challenged with a 50-fold molar excess of 29c (A) or 25c (B) oligonucleotide and incubated for 15 min with different amounts of C2-L1Tc. *29/29c and *29/25c preformed duplexes were loaded in both cases as size standards.
