FIG. 3.

Pak4 stimulates the expression of cell cycle inhibitory proteins. Immunoblots of lysates of primary MEFs (left side) and NIH 3T3 cells (right side) infected or transfected, respectively, with empty vector (E.V.), activated PAK4 (PAK4*), or RasV12. Lysates were prepared 2 days following infection. Blots were probed with the indicated antibodies. Quantifications of the protein levels relative to the empty vector control are shown. The y axis shows the fold increase over empty vector. An antiactin antibody was used as a loading control. (NIH 3T3 cells transfected with a mouse p19ARF[p19ARF] vector were used as a positive control for p19, since no background band could be seen in these cells).