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. 2005 Nov;25(21):9478–9490. doi: 10.1128/MCB.25.21.9478-9490.2005

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Copyright © 2005, American Society for Microbiology

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FIG.3. — did4Δ, vps24Δ, and vps4Δ mutations suppress rim8Δ, rim9Δ, and rim21Δ but not rim20Δ or rim13Δ. (A) did4Δ, vps24Δ, and vps4Δ mutations suppress defective Rim101 processing of rim8Δ, rim9Δ, and rim21Δ mutants but not that of rim20Δ and rim13Δ mutants. HA-tagged Rim101 was expressed from pFI1 in wild-type (TM141), rim8Δ (FM81), rim9Δ (FM91), rim20Δ (FM201), rim21Δ (FM301), and rim13Δ (MTM100) strains and their did4Δ (top panel), vps24Δ (middle panel), and vps4Δ (bottom panel) derivatives: did4Δ (VB101-6b), rim8Δ did4Δ (VB181-3a), rim9Δ did4Δ (VB191-11c), rim20Δ did4Δ (VB121-5d), rim21Δ did4Δ (VB131-1a), rim13Δ did4Δ (VB151-5d), vps24Δ (VX101-10a), rim8Δ vps24Δ (VX181-6a), rim9Δ vps24Δ (VX191-6d), rim20Δ vps24Δ (VX121-2c), rim21Δ vps24Δ (VX131-5a), rim13Δ vps24Δ (VX151-1c), vps4Δ (FT101), rim8Δ vps4Δ (FT181), rim9Δ vps4Δ (FT191), rim20Δ vps4Δ (FT121), rim21Δ vps4Δ (FT131), and rim13Δ vps4Δ (FT151) strains. Processed and unprocessed forms of HA-Rim101 (indicated) were detected as described in the legend to Fig. 1. Actin Western blotting was used as a loading control. +, intact; −, deleted. (B) did4Δ, vps24Δ, and vps4Δ mutations suppress the LiCl sensitivity of rim8Δ, rim9Δ, and rim21Δ mutants but do not suppress that of the rim20Δ or rim13Δ mutant. The strains used in panel A were streaked on YPD and YPD containing 0.32 M LiCl plates and incubated at 30°C for 5 (lower panel) or 6 (upper and middle panels) days.

FIG.3. — did4Δ, vps24Δ, and vps4Δ mutations suppress rim8Δ, rim9Δ, and rim21Δ but not rim20Δ or rim13Δ. (A) did4Δ, vps24Δ, and vps4Δ mutations suppress defective Rim101 processing of rim8Δ, rim9Δ, and rim21Δ mutants but not that of rim20Δ and rim13Δ mutants. HA-tagged Rim101 was expressed from pFI1 in wild-type (TM141), rim8Δ (FM81), rim9Δ (FM91), rim20Δ (FM201), rim21Δ (FM301), and rim13Δ (MTM100) strains and their did4Δ (top panel), vps24Δ (middle panel), and vps4Δ (bottom panel) derivatives: did4Δ (VB101-6b), rim8Δ did4Δ (VB181-3a), rim9Δ did4Δ (VB191-11c), rim20Δ did4Δ (VB121-5d), rim21Δ did4Δ (VB131-1a), rim13Δ did4Δ (VB151-5d), vps24Δ (VX101-10a), rim8Δ vps24Δ (VX181-6a), rim9Δ vps24Δ (VX191-6d), rim20Δ vps24Δ (VX121-2c), rim21Δ vps24Δ (VX131-5a), rim13Δ vps24Δ (VX151-1c), vps4Δ (FT101), rim8Δ vps4Δ (FT181), rim9Δ vps4Δ (FT191), rim20Δ vps4Δ (FT121), rim21Δ vps4Δ (FT131), and rim13Δ vps4Δ (FT151) strains. Processed and unprocessed forms of HA-Rim101 (indicated) were detected as described in the legend to Fig. 1. Actin Western blotting was used as a loading control. +, intact; −, deleted. (B) did4Δ, vps24Δ, and vps4Δ mutations suppress the LiCl sensitivity of rim8Δ, rim9Δ, and rim21Δ mutants but do not suppress that of the rim20Δ or rim13Δ mutant. The strains used in panel A were streaked on YPD and YPD containing 0.32 M LiCl plates and incubated at 30°C for 5 (lower panel) or 6 (upper and middle panels) days.