TABLE 5.
Forward mutation in LYS2-10C strains
| Strain | 10G lagging-strand template (LYS2R-10C)
|
10G leading-strand template (LYS2F-10C)
|
||||||
|---|---|---|---|---|---|---|---|---|
| Rate (10−6)a | Increase (fold)b | No. of frameshiftsc
|
Rate (10−6)a | Increase (fold)b | No. of frameshiftsc
|
|||
| −1 bp | +1 bp | −1 bp | +1 bp | |||||
| PMS1 OGG1 | 9.5 (7.3-11.6) | 1 | 1/10 | 9/10 | 19.2 (14-23) | 1 | 1/10 | 8/10 |
| pms1Δ | 1934 (886-2,981) | 203 | 17/20 | 2/20 | 4,524 (1,797-7,251) | 235 | 12/19 | 7/19 |
| pms1-G882E | 302 (153-451) | 32 | 0/20 | 20/20 | 1,316 (600-2,032) | 68 | 1/19 | 18/19 |
| pms1-H888R | 693 (350-1,037) | 73 | 0/20 | 20/20 | 1,651 (811-2,490) | 86 | 0/20 | 19/20 |
| ogg1Δ | 91 (12-170) | 9.5 | 0/12 | 7/12 | 96.5 (70-123) | 5 | 0/12 | 8/12 |
| pms1Δ ogg1Δ | 11,950 (4,310-28,220) | 1,258 | 8/13 | 5/13 | 16,570 (7,190-25,940) | 863 | 8/13 | 4/13 |
| pms1-G882E ogg1Δ | 5,587 (1,770-9,390) | 588 | 1/13 | 11/13 | 20,470 (14,360-26,570) | 1,066 | 0/13 | 13/13 |
| pms1-H888R ogg1Δ | 8,420 (5,800-11,040) | 886 | 0/13 | 11/13 | 29,100 (8,010-50,180) | 1,516 | 0/13 | 11/13 |
a
Values in parentheses are 95% confidence intervals.
b
Increases are calculated relative to PMS1.
c
Number/total number of mutants sequenced.