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. 2005 Nov;25(21):9249–9258. doi: 10.1128/MCB.25.21.9249-9258.2005

FIG. 2.

FIG. 2.

E8 enhancement of Wnt signaling is observed in various cell lines. (A) Wnt signaling in the presence of E8 was examined in Rat-1, 293, KB, and U2OS cells, as described in the legend to Fig. 1C. All the data are representative of three independent experiments that gave similar results. (B) A dual luciferase assay for Wnt signaling was carried out in the same manner as for panel A, using caspase 8−/− MEF cells. The results are representative of those obtained from more than three independent experiments that gave similar results. The expression level of E8 was confirmed by Western blotting, as for Fig. 1C. vec, empty vector.