FIG. 3.

Knockdown of Bif-1 suppresses Bax conformational change and caspase 3 activity in ADR-treated HeLa cells. (A) HeLa cells were transfected with siRNAs targeting Bif-1 or GFP for 48 h and subjected to SDS-PAGE/immunoblot analysis. (B and C) HeLa cells transfected with siRNAs targeting Bif-1 or GFP were exposed to DMSO or 1 μM ADR for 12 h and subjected to immunoprecipitation (IP) with anti-Bax 6A7 monoclonal antibody and to caspase 3 activity assay. The error bars indicate standard deviations.