FIG. 2.

NIH 3T3 fibroblast cells expressing the ubiquitination-deficient Met Y1003F receptor are tumorigenic. (A) Met Y1003F-expressing cells have an altered morphology. Phase contrast pictures of T47D cells unstimulated or stimulated for 24 h with 1.5 nM HGF. (B) NIH 3T3 cell populations expressing either wild-type Met or Met Y1003F were injected subcutaneously into nude mice. The results represent the mean tumor volume obtained from eight measurements and are representative of two independent experiments. (C) Pictures of representative tumors as well as Met protein expression in the NIH 3T3 cell populations injected and in excised tumors. The tumors were lysed in TGH buffer and total cell lysates (TCL) were blotted with Met (DL-21) antibodies. Then, the membrane was stained with Coomassie brilliant blue.