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Targeted overexpression of xPTB in the somites. The somites of GFP and xPTB-transgenic embryos were dissected, and the xPTB steady-state level in each somitic fraction was analyzed by Western blot analysis using an affinity-purified xPTB antibody. PCNA was used as a loading control. Quantification of the overall xPTB signal is shown below gels with values for GFP control embryos set as 1.
