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Nuclear-cytoplasmic RNA fractionation of TSA-treated HeLa cells by real-time RT-PCR
| Amplicon | Fractionation (relative units)
|
|
|---|---|---|
| Control siRNA | Dicer knockdown | |
| “ERV down” cytoplasmic | None | None |
| “ERV down” nuclear | 3 | 13.7 |
| “γ-Unspliced” cytoplasmic | None | None |
| “γ-Unspliced” nuclear | 0.44 | 1.9 |
| “γ-Spliced” cytoplasmic | 9.9 | 10.5 |
| “γ-Spliced” nuclear | 15.1 | 15.1 |
