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. 2005 Nov;25(21):9419–9426. doi: 10.1128/MCB.25.21.9419-9426.2005

FIG. 1.

FIG. 1.

Identification of FGF-2 interacting proteins. (a) Proteins in nuclear extracts of HeLa cells that bound to GST (lane 3), GST-FGF-2 (lane 5), or GST-FGF-2 in the presence of a 10-fold excess of hrFGF-2 (lane 6) were separated on a SDS-PAGE gel and stained with Coomassie blue. HeLa cell lysate (lane 1), purified GST (lane 2), and GST-FGF-2 (lane 4) were also separated on the gel. Numbers in lane 5 correspond to proteins bound specifically to FGF-2. (b) Proteins in lanes 1 to 6 of panel a were transferred to a polyvinylidene difluoride membrane and probed with a UBF antibody. Three bands (lane 1) in the HeLa cell lysate likely correspond to UBF1, UBF2, and phosphorylated UBF (35). (c) Purified FLAG-UBF was mixed with either GST (lane 2) or GST-FGF-2 (lane 3). Bound proteins were eluted using glutathione and then detected on immunoblots by a FLAG antibody (anti-FLAG panel) or an FGF-2 antibody (anti-FGF-2 panel). Inputs (10% of total) were loaded in lane 1.