FIG. 4.

The microarray analysis is confirmed by Northern blotting. (a) Northern blot data for the GCN4, GLC7, PGK1, PUT4, CLG1, and DBP2 mRNAs across polysomal (P) and monosomal (M) fractions from butanol-stressed (S) and control (C) yeast. For comparison, the control and stressed polysome-to-monosome ratios ([PC/MC] and [PS/MS]) as measured from the Northern blots and the microarrays are depicted on the right as histograms. (b) As for panel a, except the mRNAs GCN4, GLC7, PGK1, and PUT4 were assessed following amino acid starvation.