FIG. 5.

Comparison of motifs of the acnM translational products of several organisms with the aconitase signature. The shaded amino acids are the putative cysteine residues which bind the Fe-S-cluster (identified on the basis of similarity) (http://www.tigr.org/cgi-bin/BlastSearch/blast.cgi?organism). The boldface N is a highly conserved asparagine residue in acnM translational products; in aconitases in the tricarboxylic acid cycle this amino acid is isoleucine (indicated by boldface I). The references and accession numbers (if available), as well as contig numbers, are given in parentheses. Data for some of the organisms were obtained from the following websites: B. cepacia, R. metallidurans CH34, and P. fluorescens, http://www.expasy.ch/cgi-bin/nicesite.pl?PS00161; and P. putida KT2440, P. syringae, and S. putrefaciens, http://www.tigr.org/cgi-bin/BlastSearch/blast.cgi?organism.