TABLE 1.
cbp-1(ku258) suppression of let-60 (n1046 gf)a
| Genotypeb | Muvc (%) (n) | Inductiond (n) | Vule (%) (n) | Eglf (%) (n) | Sterilityg (%) (n) | Lethality (%) (n)
|
|
|---|---|---|---|---|---|---|---|
| Embryonich | Larvali | ||||||
| Wild type (N2) | 0 (many) | 3.0 (29) | 0 (29) | 0 (many) | 0 (many) | 1.3 (525) | 0 (518) |
| cbp-1(ku258) | 0 (233) | 2.94 (40) | 8 (40) | 8 (53) | 19 (65) | 4 (428) | 10 (411) |
| let-60(n1046 gf) | 76 (212) | 4.29 (46) | 0 (46) | 0 (42) | 0 (42) | 1.6 (613) | 1.3 (603) |
| cbp-1(ku258)/+; let-60(n1046 gf) | 10 (504) | 3.33 (33) | 0 (33) | 5 (38) | 12 (43) | ND | ND |
| cbp-1(ku258); let-60(n1046 gf) | 5 (456) | 3.18 (49) | 0 (49) | 12 (42) | 18 (51) | 13 (381) | 22 (330) |
| cbp-1(bm2)/cbp-1(bm2) | ND | 100 (many) | |||||
| cbp-1(bm2)/+ | 0 (295) | 3.0 (15) | 0 (15) | ||||
| cbp-1(bm2)/+; let-60(n1046 gf) | 83 (503) | 4.5 (26) | 0 (26) | ||||
| cbp-1(bm2)/cbp-1(ku258); let-60(n1046 gf) | 3 (380) | 3.09 (21) | 0 (21) | ||||
| tnDf2/+; let-60(n1046 gf) | 96 (307) | ||||||
| nDf20; let-50(n1046 gf); nDp2[unc-86(e1416)] | 36 (205) | ||||||
All strains were grown at 20°C. cbp-1 (bm2 lf) is linked to dpy-18 in all of the relevant strains. tnDf2 was linked to unc-32(e189). The number of animals scored (n) is indicated in parentheses. ND, not determined.
gf indicates gain of function. lf indicates loss of function.
Percentage of animals displaying one or more ectopic pseudovulvae (ventral protrusions).
Average number of VPCs adopting a vulval cell fate relative to the number in the wild-type animal, as scored with Nomarski optics. In the wild-type animal, 3 of 6 VPCs are induced.
Percentage of animals in which <3 of the VPCs normally induced in wild-type animals (P5.p to P7.p) is not induced, as scored with Nomarski optics.
Egl animals were scored as the percentage of animals that were unable to lay eggs and had a Bag phenotype (larvae that hatched within the worm). Does not include sterile animals.
Percentage of animals that reached the adult stage but were unable to produce progeny.
Percentage of eggs that failed to hatch.
Percentage of animals that died during any of the larval stages.