FIG. 3.

Tandem affinity purification of Prp43p-TAP. Total extracts from strains expressing Prp43p-TAP (lane 3) or devoid of tagged protein (lane 1) were subjected to the tandem affinity purification procedure. Polypeptides eluted from the calmodulin columns following EGTA addition were resolved by SDS-PAGE and stained with Coomassie blue. Stained proteins were excised, digested by trypsin, and identified by coupling liquid chromatography and mass spectrometry. Lane 2: molecular mass markers (in kilodaltons).