FIG. 3.

Development of T and B cells in Sly1^d/d mice. (a) Thymocyte subsets were analyzed by flow cytometry using antibodies against CD4 and CD8. (b) Subsets of double-negative thymocytes were further differentiated by their CD44 and CD25 expression profile (upper panels). In the lower panel, results from eight to nine independent mice per group are summarized. (c) Histograms depict surface levels of T-cell receptor β(TCRβ) and CD5 on double-positive (DP) and single-positive (SP) thymocytes (wild type [WT], shaded graphs; Sly1^d/d mice, black lines). (d) Sly1^d/d mice were mated with mice expressing a transgenic T-cell receptor specific for the HY antigen in the context with H2-D^b. Thymocytes were gated for HY-TCR^high cells and the proportions of CD4- and CD8-defined subsets present in female and male mice are shown. (e) The proportions of CD4 and CD8 T cells (gated on CD3⁺ cells) and (f) CD21^hi CD23^lo (gated on IgM⁺ cells) or IgM^hi IgD^lo marginal zone B cells in spleens of wild-type and Sly1^d/d mice were determined. All plots are representative of at least 6 independent mice per group. #, P < 0.01 (Student's t test).