FIG. 1.

Glut4 protein (A), basal glucose transport (B), and glycogen content (C) levels in muscle from WT and muscle-G4KO mice. Mice were fasted overnight. (A) Proteins in muscle lysates (25 μg) were separated by SDS-PAGE on 10% gels and transferred to nitrocellulose membranes. Glut4 was visualized by immunoblotting with a Glut4 antibody. (B) Isolated soleus muscles are incubated with 2-deoxy-d-glucose for 20 min. Basal glucose uptake was determined. Results are means ± SEM for three to six mice per group. **, P < 0.01 versus WT. (C) Soleus, gastrocnemius, and tibialis anterior muscle specimens were solubilized with 0.5 N KOH. The glycogen content levels in muscle of WT and muscle-G4KO mice at 4 months of age were determined by use of a glucose oxidase method. Results are means ± SEM for five to six mice per group. *, P < 0.05 versus WT.