FIG. 8.

Glycogen content levels (A), glycogen phosphorylase activity levels (B), and PTG protein amount (C) in liver from WT and muscle-G4KO mice. Mice were fasted overnight. (A) Liver was solubilized with 0.5 N KOH. The glycogen content was determined by use of a glucose oxidase method. (B) The ratio of glycogen phosphorylase activity represents the activity measured in the absence divided by that measured in the presence of AMP. (C) Proteins in liver lysates (25 μg) were separated by SDS-PAGE on 10% gels and transferred to nitrocellulose membranes. PTG were visualized by immunoblotting with a PTG antibody. Results are means ± SEM for five to eight mice per group. OD, optical density.