TABLE 1.
Pigmentation scores in (E)(Y)W, (E)(Y)SW, and (E)(Y)WS transgenic lines and their transheterozygous enhancerless and promoterless derivatives
| Transgenic line (localization) | Cuticle pigmentation score (wing/body)a
|
|||
|---|---|---|---|---|
| (E)(Y)/+ | (ΔE)(Y)/(E)(ΔPRY)
|
|||
| +/+ | su(Hw)− | mod(mdg4)ul | ||
| (E)(Y)W | ||||
| 1 (3) | 5/4 | 3-4/3-4 | ND | ND |
| 2 (3) | 4-5/3-4 | 1/2-3 | ND | ND |
| 3c (3) | 4/4 | 3-4/3 | ND | ND |
| 4 (3) | 3-4/2-3 | 3-4/1-2 | ND | ND |
| 5 (3) | 3-4/2 | 2/2 | ND | ND |
| 6 (3) | 4/3-4 | 2-3/2 | ND | ND |
| 7 (3) | 4/3 | 3-4/4 | ND | ND |
| 8 (2) | 4/2-3 | 3/3 | 3/2-3 | 3/2 |
| 9 (3) | 3/2-3 | 1/2 | ND | ND |
| 10 (2) | 4-5/3-4 | 2/2 | 2/1-2 | 2/2 |
| 11 (3) | 4-5/4-5 | 1/2 | ND | ND |
| 12 (2) | 5/3-4 | 2-3/2 | 2-3/2 | 2/2 |
| APSb | 4.1/3.3 | 2.4/2.5 | ND | ND |
| (E)(Y)SW | ||||
| 13 28E9 | 5/5 | 3-4/3 | 2/2 | ND |
| 14 64B12 | 4-5/4 | 4/3-4 | ND | ND |
| 15 13A5 | 4-5/4 | 4-5/4 | 3-4/2-3 | ND |
| 16 (2) | 4/3-4 | 3-4/3-4 | 2/2 | ND |
| 17 52B2 | 4-5/4 | 3-4/3 | 2/2 | 2/2 |
| 18 64C9 | 3-4/3-2 | 3-4/3 | ND | ND |
| 19 (2) | 4-5/5 | 4-5/3-4 | 3/3 | 3-4/3 |
| 20 58Fd | 4/3-4 | 2-3/2-3 | 1-2/1-2 | ND |
| 21c (2) | 2-3/3 | 2-3/3 | ND | ND |
| 22 (3) | 5/4-5 | 5/4-5 | ND | ND |
| APS | 4.2/3.9 | 3.7/3.4 | 2.3/2.2 | ND |
| (E)(Y)WS | ||||
| 23 20E | 3-4/3-4 | 3/2 | ND | ND |
| 24 (3) | 4/3-4 | 4/3 | ND | ND |
| 25 23A3 | 4/3 | 3-4/3 | 1-2/1-2 | 3-4/2 |
| 26 39E3 | 5/5 | 5/4-5 | 4/3 | 5/4 |
| 27 29D4 | 5/5 | 5/3-4 | 3/2 | 3-4/2 |
| 28 75B5 | 4-5/4 | 4/3-4 | ND | ND |
| 29 100B3 | 5/4 | 4/3 | ND | ND |
| 30 68C13 | 4/3 | 3/2-3 | ND | ND |
| 31c 74A1 | 4/3 | 4/3 | ND | ND |
| 32 35DEd | 4/4 | 3-4/3-4 | 1/1 | 3-4/3-4 |
| 33 (3) | 4-5/4 | 3-4/3 | ND | ND |
| 34 28D2 | 4-5/4 | 4-5/3 | 3/2 | 4-5/3 |
| APS | 4.3/3.8 | 3.9/3.1 | 2.5/1.9 | 3.8/2.9 |
Pigmentation levels are shown for heterozygous (E)(Y)WS, (E)(Y)SW and (E)(Y)W females [(E)(Y)/+] and for transheterozygous females carrying the promoterless and enhancerless derivatives of the constructs on the +/+, su(Hw)−, or mod(mdg4)ul background [(ΔE)(Y)/(E)(ΔPRY)]. Bold data indicate complementation between y alleles. ND, not determined.
APS, average pigmentation scores assigned to each transgenic construct. Statistical data are available at http://www.igb.ac.ru/Kravchenko-Suppl.pdf.
Flies in these transgenic lines displayed variegated pigmentation of bristles.
Localization of these insertions was determined by FISH.