. 2002 Jan;68(1):124–137. doi: 10.1128/AEM.68.1.124-137.2002

TABLE 1.

Primers used for amplification and sequencing of 16S rRNA genes

Primer designation	Sequence	Use(s)	Reference(s)
968F-GC	CGCCCGCCGCGCCCCGCGCCCGTCCCGCCGCCCCC GCCCGAACGCGAAGAACCTTAC	TTGE analysis	30
1401R	CGGTGTGTACAAGACCC	TTGE analysis	30
8FPL	AGTTTGATCCTGGCTCAG	Cloning	35 ^a
1492RPL	GGYTACCTTGTTACGACTT	Cloning	35 ^a
515FPL	AGTGCCAGGMGCCGCGG	Cloning	35 ^a
63F	CAGGCCTAACACATGCAAGTC	Cloning, sequencing	22
1387R	GGGCGGWGTGTACAAGGC	Cloning, sequencing	22
1387R-AC	ACGGGCGGWGTGTACAAGGC	Cloning	22, this study
1391R	GACGGCGGTGTGTRCA	Detection of archaea	9, 35
23FPL	GCGGATCCGCGGCCGC	Detection of archaea	9
A109F	ACKGCTCAGTAACACGT	Detection of archaea	19, 53
A934R	GTGCTCCCCCGCCAATTCCT	Detection of archaea	19, 53
361F	GGAATATTGGACAATGGGC	Sequencing	54 ^b
379R	GCCCATTGTCCAATATTCC	Sequencing	54 ^b
522F	CAGC(A/C)GCCGCGGTAAT(A/T)C	Sequencing	54 ^b
536R	G(A/T)ATTACCGCGGC(G/T)GCTG	Sequencing	54 ^b
750F	CTGACGCTGAGGAGCGAAAG	Sequencing	54 ^b
769R	CTTTCGCTCCTCAGCGTCAG	Sequencing	54 ^b
927F	GGGCCCGCACAAGCGGT	Sequencing	54 ^b
943R	ACCGCTTGTGCGGGCCC	Sequencing	54 ^b
1075F	TCGTGAGATGTTGGGTTAAG	Sequencing	54 ^b
1094R	CTTAACCCAACATCTCACGA	Sequencing	54 ^b

Primers based on this reference, but sequences introducing restriction sites were omitted. Primer 515FPL was synthesized such that base number 10 was either A or C.

Sequencing primers based on this reference, but with modifications to improve primer efficiency.