FIG. 4.

vps11 mutants are defective in differentiation. (A) Cell suspensions of each strain were applied as spots to M199 agar and incubated at 37°C. Images were taken at day 4. (B) Each strain was induced to filament in 10% FCS liquid culture at 37°C. Images were taken after 5 h of incubation. (C) Chlamydospore formation was assessed on cornstarch Tween agar, with incubation in the dark. Arrowheads indicate apparently mature chlamydospores, and an arrowhead with a question mark indicates a putatively immature chlamydospore.