TABLE 2.

Primers used in this study

Primer	Sequence (5′→3′)	Purpose
11R5	TCAGGATCCAGGCAATACATGTGATTCG	Amplification of VPS11 and flanking sequences
11R3	TCAGGATCCATTGGGAGTCCCCCCACTCC
11SEQF	CCGTTGCATTCAATTTGGGCC	PCR analysis and sequencing of heptad repeat region of VPS11
11SEQR	CTTTTCATATTTGGCATTGGG
HIS1268F	CCGCTACTGTCTCTACTTTG	Detection of HIS1 integration
URA3-5	CCTATGAATCCACTATTGAACC	Detection of URA3 integration
11HISR	ACTGGGTGCTAGTTCTTTCC	Detection of VPS11 sequences at HIS1 locus
HIS3AMP	GTTGGTGTGGCCCAGAGAC