FIG. 1.

HDAC activities of A. nidulans wt and hdaA deletion strains. (A) Elution profile of HDAC activity after Source 15Q anion-exchange chromatography of protein extracts of A. nidulans wt (gray line) and ΔhdaA (black line) strains. Elution was performed with 50 ml of a linear gradient (dashed line) of 10 to 500 mM NaCl in buffer B at a flow rate of 1 ml/min. Fractions of 1.5 ml were collected and assayed for HDAC activity. Fraction 26 of the ΔhdaA strain, used for subsequent immunoprecipitation with an RpdA antibody, is underlined. Fractions used for immunoblotting are shown in boldface. (B) Immunological detection of HdaA in Source Q fractions 18 to 30. Aliquots of each fraction were subjected to SDS-PAGE with subsequent blotting onto nitrocellulose membranes. A specific antibody against HdaA was used for immunodetection. The fraction with the main HDAC activity is shown in boldface. All data shown in this figure are derived from H4 as the ΔhdaA strain and H1 as a control representing the wt. As a loading control, aliquots used for immunodetection were stained with Coomassie blue (lower panel).