FIG. 4.

Eng1p glucanase activity is not dependent on rho4⁺ levels. β-1,3-Glucanase activity was assayed in extracts from wild-type (wt), eng1Δ, rho4Δ, and rho4⁺-overexpressing cells (A); in extracts from wild-type, rho4Δ, and rho4⁺-overexpressing cells containing pREP3Xeng1⁺ in the presence (hatched bars) or absence (black bars) of thiamine (B); and in culture supernatants of wild-type, eng1Δ, and rho4Δ cells grown at 37°C (C). Values are the means from at least three independent experiments. Error bars represent the standard deviations.