FIG. 1.

Growth defects of C. albicans formin mutants during yeast growth. Growth of the wild type and formin mutant strains was monitored using time-lapse microscopy over several hours (timescale is in hours:minutes) (A-C). In the movie of the wild type at http://pinguin.biologie.uni-jena.de/phytopathologie/pathogenepilze/index.html, a characteristic change of cell axes after cytokinesis can be observed (previous mother-bud axes are indicated as white, dotted lines and newly established axes as black, dashed lines in panels A to C). This results in lateral movement of cells such that wild-type colonies form a single cell layer (A). In the bni1 mutant, growth was irregular and cells were dispatched in three dimensions. A shift of mother-bud axes occurs but often only due to mechanical forces generated by new buds. The arrows denote enlarged septal sites (B). In the bnr1 mutant, growth axes are kept over several cell cycles, resulting in a linear array of mother and daughter cells (C). Cell shape and budding pattern of the indicated strains were analyzed after staining the cells with calcofluor (D). wt, wild type. Scale bar, 5 μm.